Technical note forensic science communications october 2004. Eleven step of software testing process slideshare. A drop of phenolphthalin reagent is added to the sample, and after a few seconds, a drop of hydrogen peroxide is. The presumptive blood test forensics demonstration kit utilizes a simulation of the kastlemeyer color test and can be safely used in a mock crime scene investigation.
We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. Looking for inspiration for a science fair project. The kastlemeyer km test is a presumptive test for blood that is widely used in forensic science. Effective this date, this administrative manual version 3. Chemical used in the kastlemeyer test which is a substrate for the perioxsidaselike chemicals found in hemoglobin. Kastlemeyer km presumptive testing for blood status. This test relies on the peroxidaselike activity of the heme groups associated with the hemoglobin contained in red blood cells. Study 602 forensic bio study guide 20 14 hughes stamm flashcards from studyblue on studyblue. In addition to the existence of crimes against the person, there is an increasing number of complaints around the world due to mistreatment of animals.
Phenolphthalin does not directly participate in this process. Evaluation of six presumptive tests for blood, their specificity, sensitivity, and effect on high molecularweight dna. The topic selection wizard tool can help you find a project you will enjoy. For example, it cannot tell us who the blood belongs to, or even if it is from a human. Kastle meyer km forensic test for the presence of blood. Forensic techniques encompass those procedures employed. It relies on the peroxidaselike activity of hemoglobin in red blood cells to catalyze the oxidation of the colorless reduced form of phenolphthalin into phenolphthalein, which is visible as bright pink color. The kastlemeyer test is a presumptive blood test, first described in 1903, in which the. You will need to order the presumptive blood test kit online.
The kastlemeyer test is a good forensic tool, but it does have its limitations. Greg taylor and darryl hunt programs on april 12, 2012, 4102012. In this study, blood dilution sets were created with concentrations from neat to 1. Presumptive test for blood keep refrigerated packaged in 100 ml, 500 ml and 1 l quantities. If the test result is negative, it is reasonable proof that heme an ingredient in all blood is absent in the sample. The presumptive blood test forensics demonstration kit utilizes a simulation of the kastle meyer color test and can be safely used in a mock crime scene investigation. The kastlemeyer test is a simple, reliable and inexpensive test to detect blood. Another limitation is that the kastle meyer test will react with any hemoglobin based blood sample, which could be human or animal. Repeat steps 16 using the nail file rather than the letter opener. Jan 28, 2014 the kastle meyer test is a good forensic tool, but it does have its limitations. In the presence of hydrogen peroxide, this peroxidaselike activity will catalyze the oxidation of phenolphthalin, which is colorless in solution, into. Many analytical chemists focus on a single type of instrument. Kastlemeyer test this laboratory protocol or part thereof has been provided as an example of a laboratory sop, courtesy of the illinois state police. Presumptive blood testing the purpose of this lab is to give you practical experience in the method of applying various presumptive blood testing kits to suspected stains.
Each dilution set was subjected to bluestar and to the kastle meyer test consecutively. Phenolphthalein will cause an alkaline solution to turn pink after its oxidation by peroxide when blood is present. The 3stage kastle meyer km test has been used for decades as a rapid, presumptive test for blood. The body fluid is located using a screening test and possibly a confirmatory one. Ncjrs abstract national criminal justice reference service. Investigate the chemistry of the kastlemeyer test for the presence of blood. This test is inexpensive, easy to perform, and provides quick results. The kastlemeyer color test is commonly used to presumptively indicate if blood is present on an object. A presumed blood sample is first collected with a swab.
Presumptive test for blood kastlemeyer test procedure. The kastle meyer test is applied to samples that have been subjected to chemical blood reagents in the field. Dna analysis part 1 introduction to forensic science. In this crime scene chemistry science project, you will learn how to perform the kastlemeyer test for blood, and you will test various substances to see which others also give a positive test result. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Noncatalytic confirmatory blood tests, for example, hexagon obti, hemeselect.
Forensic biology social science flashcards quizlet. Forensic bio study guide 2014 hughes stamm instructor. Also your steps listed above while probably not the cause of your. Presumptive tests for blood play a critical role in the examination of physical evidence and in the determination of subsequent analysis. Simply wipe a presumptive bloodstain with a cotton swab, add a drop of the reagent, and then apply a drop of hydrogen peroxide. The kastlemeyer km test is a quick and easy chemical test for blood used in forensic analyses. The kastlemeyer test reducing phenolphthalein the kastlemeyer test is a test used in forensics to detect blood, whereupon blood is swabbed, the phenolphthalein solution is added, followed by the addition of hydrogen peroxide. Pdf evaluation of six presumptive tests for blood, their. Each step is marked pass or fail based on the comparison result between the expected and actual outcome. The detection reagent is phenolphthalin, where it is reduced phenolphthalein. This article describes and evaluates alternative forensic procedures that were used to test for the presence of blood in a red inscription inside of the lid of an antique wooden memento box that is attributed to the property of and is inscribed and signed frida kahlo. The kastlemeyer blood test is an extremely sensitive test, capable of detecting blood dilutions as low as 1. The late 20th century also saw an expansion of the application of analytical chemistry from somewhat academic chemical questions to forensic, environmental, industrial and medical questions, such as in histology.
A wipe may be made using a piece of filter paper, thread, or swab. The kastlemeyer test is an inexpensive, easy, and reliable forensic method to detect the presence of blood. This bibliography was generated on cite this for me on sunday, april 9, 2017. The reagent does not only serve as a useful test for forensic scientific. Further genetic and forensic tests need to be carried out in order to find the answers to those questions.
In the 2step version of the test, the kastlemeyer reagent is mixed only with an. It relies on the peroxidaselike activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin the colorless reduced form of phenolphthalein into phenolphthalein, which is visible as a bright pink color. Moisten a swab with water and touch it to a dried blood sample. The kastle meyer test is a simple, reliable and inexpensive test to detect blood.
If the swab turns pink rapidly, it is positive for blood. In the test s final step, the sample is treated with hydrogen peroxide, which reacts with hemoglobin and phenolphthalein to produce a bright pink colour. Hey guys i did the kastle meyer test on a sample of my own dried blood. Notably, the results of such a test are not at all inculpatory of any defendant. This laboratory protocol or part thereof has been provided. The kastlemeyer test has the same reaction with human blood as it does with any other hemoglobinbased blood, so a confirmatory test such as the ouchterlony test must be performed to definitively conclude from which species the blood originated.
This is an application developed, owned, and operated by kastle systems. All of the reagents are supplied in plastic dropper bottles for easy use. Positive reactions were obtained from all unstained areas of the fabric tested. Contact antec to discuss your chemical and equipment needs. The heme group in blood catalyses the decomposition of h. Classic test for the presence of blood at a crime scene. Perform a presumptive blood test using the kastlemeyer test to check for blood on materials. It cannot tell us how long the blood has been there, or the blood group.
Teaching activities teaching science blood test dna test forensics science. After the visualization assay was performed, fabrics and filter papers were dried in a fume hood upon which the presumptive blood tests were performed on both the blood spot on the. Hold swab over the plastic waste dish and apply 1 drop of ethanol from the dropper bottle to the swab. Here is how to prepare the solution used for the forensic test. Wet the wipe with water, then rub over the stained area while still wet. Color catalytic tests are very sensitive, but not specific. Kastle meyer km test is a quick, simple, reliable, and not expensive forensic test for the presence of blood, its sensitive, specific, and consume as little of the sample as possible. In the tests final step, the sample is treated with hydrogen peroxide, which reacts with hemoglobin and phenolphthalein to produce a bright pink color. Moreover, the visualization test was evaluated in combination with a presumptive blood test kastlemeyer test 17, 18, lumiscene, 7 and fluorescein test 3, 6. The kastle meyer test is a form of catalytic blood test, one of the two main classes of forensic tests commonly employed by crime labs in the chemical identification of blood. In the kastlemeyer test, the chemical phenolphthalein is used to detect blood because phenolphthalein can change color if it comes in to contact with certain chemicals that are found in the blood. Fbis program of support for criminal justice dna database plus the software used to run these. The phenolphthalin reagent kastlemeyer reagent was prepared by reducing phenolphthalein with zinc metal in basic solution and an approximately 20percent sodium perborate solution was used in.
Never consume your entire sample with a presumptive test. The other class of tests used for this purpose are microcrystal tests, such as the taichmann crystal. Science buddies has over 1,200 project ideas in all areas of science. It relies on the peroxidaselike activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin the colorless reduced form of phenolphthalein into phenolphthalein, which is visible as a bright pink. The kastlemeyer test is a presumptive blood test, first described in 1903, 1 in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin.
The phenolphthalin reagent kastle meyer reagent was prepared by reducing phenolphthalein with zinc metal in basic solution and an approximately 20percent sodium perborate solution was used in. The kastlemeyer test for the detection of blood the bmj. The kastlemeyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. Sensitivity and specificity of presumptive tests for blood. Such methods are typically based on the detection of hemoglobin blood through a phenolphthalein kastlemeyer test and the detection of saliva through an. Hey guys i did the kastle meyer test on a sample of my own dried blood and did not work. This test method is designed for the guidance of laboratory personnel who assist. The authors shown below used federal funds provided by the u. The dna profile obtained from the bloodstain on the suspects shirt matches the dna profile obtained from the victim. Add a boiling chip and gently boil the solution until it changes color to become colorless or pale yellow. Rub surface of letter opener suspected of blood with swab 45 times. Wear safety goggles and disposable gloves for this procedure. However, the test will give a false positive result. The test provides evidence if red spots found at a crime scene are actually blood.
A pair of jeans produced false positive results upon testing for the presence of blood using the kastle meyer km test. One of the classic tools is the kastlemeyer test for the presence of blood. There is a lot of information on the kastlemeyer test on wikipedia here. Get a printable copy pdf file of the complete article 298k, or click on a page image below to browse page by page. Technical note forensic science communications october. A simple chemical testfor blood, it is the kastlemeyer test, which indicates the presence or absence of ironresolves whether testing should continue.
Let us say that we need to check an input field that can. Therefore, the use of a qc test procedure to check the reliability of the reagent prior to its use in casework is not an absolute requirement, but. While developing the test cases, we usually have the following fields. You do not need to rub hard or coat the swab with the sample.
Body fluid identification national institute of justice. Kastlemeyer reagent, 100 ml, forensic test flinn scientific. Sciencemadness discussion board the kastlemeyer test. Perform the classic 3step kastle meyer test for blood using the premixed solutions in this convenient kit. Blood detection by chemical methods nz institute of chemistry. There is a lot of information on the kastle meyer test on wikipedia here. You would only do a presumptive test if your sample is large enough. The kastlemeyer test is a form of catalytic blood test, one of the two main classes of forensic tests commonly employed by crime labs in the chemical identification of blood. The kastle meyer test is an inexpensive, easy, and reliable forensic method to detect the presence of blood. Kastlemeyer test and its application in medical sciences. The other class of tests used for this purpose are microcrystal tests, such as the teichmann crystal test and the takayama crystal test. Peroxidases are a class of enzymes that catalyze the oxidation of compounds in the presence of a peroxide.
A very popular presumptive catalytic method is the phenolphthalein test which is also known as the kastle meyer test. Kastlemeyer test is a forensic presumptive blood remain test. Department of justice and prepared the following final report. Full text full text is available as a scanned copy of the original print version. Evaluation of a visualization assay for blood on forensic evidence. First, plant evidence in the crime scene using the included blood standard strips. I swabed the blood with a moist swab, added a drop of a solution of phenolpthalein, ethanol, and methanol, then added one drop of hydrogen peroxide. The luminol test and kastle meyer tests are both presumptive tests, meaning that a positive result only tells that it is possible there is blood in the sample.
Phenolphthalein falsepositive reactions from legume root. Formed when blood falls onto a lessthansmooth surface forming this pattern. Moisten with distilled water green color luminol test. Test steps describe the execution steps and expected results that are documented against each one of those steps.
Phenolphthalein is a presumptive test that reacts with the heme molecule present in blood. In the kastle meyer test, the chemical phenolphthalein is used to detect blood because phenolphthalein can change color if it comes in to contact with certain chemicals that are found in the blood. Hemoglobin has a peroxidase activity, which forms the basis of the km test. It is fast and easy to use, making it an ideal test at a crime scene or for use on samples taken from a crime scene. Kastlemeyer blood test reagents are deleterious to dna. Using the provided premixed solutions, test samples to see if they react. For example, in the report below it is not clear what blood analysis tests were. Two practical variations of this test are the kmrub indirect test and the more sensitive km. Distilled water, ethyl alcohol, phenolphthalein, hydrogen peroxide. You do your presumptive blood test using the kastlemeyer test. If you continue browsing the site, you agree to the use of cookies on this website. Kastlemeyer presumptive testing for blood effective 04. It includes all the premixed chemicals and supplies required to conduct the phenolphthalein blood test.
Kastlemeyer reagent contains reduced phenolphthalein indicator in a basic solution. Phenolphthalein kastlemeyer reagent, presumptive test for blood. Our phenolphthalein presumptive blood test kit is packaged in a durable, lightweight plastic box for easy and safe transport from one crime scene to another. It has been included for training and example purposes only. Kastlemeyer test is very effective in detecting latent blood, mainly due to its sensitivity. More information the kastle meyer test is an inexpensive, easy and reliable forensic method to detect the presence of blood. Presumptive blood testing lab kastlemeyer test 201617 ivc spring. Perform the classic 3step kastlemeyer test for blood using the premixed solutions in this convenient kit.
Modern analytical chemistry is dominated by instrumental analysis. Km test is a forensic pretest for the indication of suspected traces of blood. The probability of a randomly selected, unrelated individual having the same dna profile as the victim and the bloodstain on the suspects shirt is less than 1 in 4. You use the swab to find out whether the stains are indeed blood. The catalytic power of hemoglobin allows colorimetric reactions employing phenolphthalein kastle meyer test to indicate whether blood is present. However, the test will give a false positive result in the presence of an oxidizing agent in the sample. Evaluation of a visualization assay for blood on forensic. It is intended for authorized users only for conducting business activities. The kastle meyer test has the same reaction with human blood as it does with any other hemoglobinbased blood, so a confirmatory test such as the ouchterlony test must be performed to definitively conclude from which species the blood originated. Kastlemeyer phenolphthalein reagent in dropper bottles 100 ml of distilled water, 2 g of phenolphthalein, 20 g of koh, and 10 g of zinc dust. Get a printable copy pdf file of the complete article 929k, or click on a page image below to browse page by page.
These are the sources and citations used to research kastle meyer test. We are continually educating our customers and the industry as a whole with relevant product information, articles, webinars, demos and more. This suggests the presumptive tests had no impact on the ability of the test to provide a result. A drop of phenolphthalein reagent is added to the sample, and after a few. Dec 03, 20 eleven step of software testing process slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Results after the preparation of the kastle meyer reagent and the three day wait the tests were started. Kastle systems has been an innovative leader in the security industry for more than 40 years. I very blur with this, and i do not know whether if i use the pure phenolphthalein is work.
1378 959 797 1172 305 961 587 102 1183 206 631 845 441 387 1096 132 1544 1461 327 1539 121 731 109 1438 49 722 473 492 894 1443 1376 929 777 1007 803 26